fixative for eyes
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From: | Snobird75@aol.com |
To: | Histonet@pathology.swmed.edu |
Reply-To: | |
Content-Type: | text/plain; charset="US-ASCII" |
Hello
Our lab uses a fixative called Davidson's Fixative
95% ethyl alcohol 30ml
10% neutral buffered formalin 20ml
Glacial Acetic Acid 10ml
Distilled water 30ml
+ you can make as much as you want useing the amount above. We use 10 times as much.
*Tissue must be removed from the fixative by 24hrs
then we rinse the tissue in the sink and put into 10% formalin, then process it on the tissue processor.
**There is a note added to where you would transfer the tissue into 70% alcohol and refrigerate.
but we don't do this step.
** this fixative has been used for research animal, don't know what would happen if you are using human eyes.
I hope this helps
sandi miller
gov't research
maryland
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