Re: wanted, if possible, Paragon stain

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From:Shirley Powell <powell_sa@Mercer.EDU>
To:Gayle Callis <>
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Hi Gayle,

Back when I worked at a hospital Paragon stain, which we used for frozen
sections,  became scarce if not extinct. I found a reference for the stain,
which I modified, and made it up myself.  The following is what I have on a
procedure card from that era.  Hope this helps, we used it for frozen sections
for years:

Modified Polychrome Stain - Ref:  Barlow, Wm, HT(ASCP) Riverside Hospital,
Wilmington, Delaware - modified by Shirley Powell, HT(ASCP)HTL, QIHC, Mercer
University School of Medicine

35% Methyl alcohol            500 mL
Toluidine blue W                     7 gm
Basic Fuchsin                          3 gm
Mix well and store at room temperature

Cut frozen sections and fix in methanol.  Remove and flood sections with
stain,  rinse in tap water.  Sections may be coverslipped with aqueous
mounting media at this point or may be dehydrated in graded alcohols, cleared
in xylene or substitute and coverslipped.

If you have more questions call me at 912-301-2374.

Shirley Powell

Gayle Callis wrote:

> Is the Paragon stain made anymore?   Supplier?  Is there a recipe for this
> staining solution?
> Gayle Callis

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