Regarding Adriana's question about which cytokeratin to use on 
sentinel nodes:  Look at the abstract (#1321) by Linden and Zarbo in 
Modern Pathology 2000; 13:224A.  They compared AE 1/AE3, CAM 5.2 and a 
CK cocktail of 7 anti-CK Abs.  They found the CK cocktail to be the 
mosts sensitive Ab, staining more tumor cells with increased 
intensity.

Bruce A. Schainker, M.D.
Director, Department of Pathology
Holy Name Hospital
Teaneck, NJ



------------------ Reply Separator --------------------
Originally From: "Turriago, Adriana" <ATURRIAGO@chromavision.com>
Subject: sentinel node
Date: 02/07/2000 01:41pm


Hi all!

Could anyone tell me which Ab(s) is (are) most commonly used in 
sentinel
node staining?  Which one is better and gives the least amount of
background?  Is there a great difference between AE1/AE3 and CAM 5.2?

Thanks for your help

Adriana