Re: keratin cocktails

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From:amos brooks <>
To:Colleen Forster <>
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Hi Colleen,
    Thanks for the info regarding the cytokeratin. How would you deal with
antibodies requiring different pretreatment (e.g. PK and HIER)? Also is
there any documentation for this mixing. I dont doubt you, I would just like
to have a reference to point out to management and doctors so they dont fear
the inquisition ... (inspectors).
Amos Brooks

Colleen Forster wrote:

> Amos,
> You make cocktails of the different MW keratins to stain a full range on
> one piece of tissue. I am sure you know that different MW cover
> different Moll numbers of the keratins. The full range covers basic and
> acidic so cocktails are designed to hit a specfic target range of Moll/
> MW. I have worked at 2 different labs where alot of thought was put into
> the needs of the cocktail and keratins researched to see what would be
> the best combination. Then the titres are figured out individually first
> to learn the staining pattern and then mixed into a cocktail for a full
> result. This technique is used quite alot.
> Colleen Forster
> U of MN, Dept. of Neurology

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