Re: counterstain
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From: | "J. A. Kiernan" <jkiernan@julian.uwo.ca> |
To: | Lynn Gardner <lynn-gardner@uiowa.edu> |
Reply-To: | |
Content-Type: | TEXT/PLAIN; charset=US-ASCII |
On Wed, 2 Feb 2000, Lynn Gardner wrote:
> Does anyone have a green counterstain that is not a nuclear stain that can
> be used for immuno and it works consistantly?
You didn't identify the immuno end-product. If it's oxidixed
DAB (brown) then the obvious non-nuclear green counterstains
are light green and fast green FCF. The latter is better for
permanent preparations. Either should be applied from a slightly
acidified solution and the sections should then be treated
like any others stained by an anionic (acid) dye: see any
textbook, or recall HistoNet messages sent from many, to all
and often. [There should be a 4-word Latin motto there, but
I'm too dim. Any offers?]
John A. Kiernan,
Department of Anatomy & Cell Biology,
The University of Western Ontario,
LONDON, Canada N6A 5C1
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