Re: counterstain

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From:"J. A. Kiernan" <>
To:Lynn Gardner <>
Content-Type:TEXT/PLAIN; charset=US-ASCII

On Wed, 2 Feb 2000, Lynn Gardner wrote:

> Does anyone have a green counterstain that is not a nuclear stain that can
> be used for immuno and it works consistantly?

  You didn't identify the immuno end-product. If it's oxidixed
  DAB (brown) then the obvious non-nuclear green counterstains
  are light green and fast green FCF. The latter is better for
  permanent preparations. Either should be applied from a slightly
  acidified solution and the sections should then be treated 
  like any others stained by an anionic (acid) dye: see any
  textbook, or recall HistoNet messages sent from many, to all
  and often. [There should be a 4-word Latin motto there, but
  I'm too dim. Any offers?]

 John A. Kiernan,
 Department of Anatomy & Cell Biology,
 The University of Western Ontario,
 LONDON,  Canada  N6A 5C1


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