On Wed, 9 Feb 2000, Hooker, Anna wrote:

> Does anybody have a good recipe for citrate buffer pH 6?

  Here's one from 2 concentrated stock solutions.
  Be careful to check if the citric acid is anhydrous
  or monohydrate crystals.

  2M sodium hydroxide  (= 8.0% NaOH):   28.0 ml
  2M citric acid (= 38.2% anhydrous
        H3Cit, _or_ 42.0% H3Cit.H2O):  10.0 ml
  Water:                       to make 100 ml

  This has pH 6.0 at 20C. The pH falls with higher
  temperature (5.9 at 50C).

  Source: Perrin,DD & Dempsey,B (1974) Buffers for pH
  and Metal Ion Control. London: Chapman & Hall. p.130.
  (This little paperback - 176 small pages - is an
  excellent reference for buffers.)

 John A. Kiernan,
 Department of Anatomy & Cell Biology,
 The University of Western Ontario,
 LONDON,  Canada  N6A 5C1
   Phone: (519) 661-2111
   FAX (Department): (519) 661-3936
   E-mail: kiernan@uwo.ca