Re: Twort Stain
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From: | Kathy Wortham <kjwortham@yahoo.com> |
To: | CEJones0476@aol.com |
Reply-To: | |
Content-Type: | text/plain; charset=us-ascii |
--- CEJones0476@aol.com wrote:
> Does anyone use the Twort stain by Newcomer Supply?
> If you haven't heard of
> this procedure it is a variation of the Brown and
> Hopps that eliminates the
> use of picric acid acetone. The lab I work for
> switched to this procedure for
> various reasons, but we are having problems with
> consistency and the
> pathologists are not satisfied. Although the gram
> negative and gram positive
> bacteria are staining appropriately, the background
> has never been light
> green as the results state. If you have any
> suggestions or other methods that
> could be tried it would be really appreciated.
> Thanks in advance,
> Charnell Jones, HTL(ASCP)
> Compunet Clinical Laboratories
> Dayton, OH
>
>
Hi Charnell,
We make up our own Twort for our Lillie-Twort
modified gram stain.
We have increased the strength of the stain from the
original procedure(Pathologists preference) so you may
need to adjust.
Modified Twort stain--Stock original
Neutral red (C.I. #50040) 0.6gm (0.36gm)
Fast green (C.I. #42053) 0.2gm (0.04gm)
Mix with 200ml 100% ETOH
Procedure says stock solution is good for 1-3 months.
I don't agree, I make up fresh each time I need to
make more working solution (once a month)
Twort working solution
1 part stock: 2 parts distilled water
Filter AFTER dilution. Ready to use.
=====
Kathy Wortham
USDA,Food Safety Inspection Service
950 College Station Road
Athens, Ga 30605
(706)546-3556
kjwortham@yahoo.com
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