Re: Twort Stain

<< Previous Message | Next Message >>
From:Kathy Wortham <kjwortham@yahoo.com>
To:CEJones0476@aol.com
Reply-To:
Content-Type:text/plain; charset=us-ascii



--- CEJones0476@aol.com wrote:
> Does anyone use the Twort stain by Newcomer Supply?
> If you haven't heard of 
> this procedure it is a variation of the Brown and
> Hopps that eliminates the 
> use of picric acid acetone. The lab I work for
> switched to this procedure for 
> various reasons, but we are having problems with
> consistency and the 
> pathologists are not satisfied. Although the gram
> negative and gram positive 
> bacteria are staining appropriately, the background
> has never been light 
> green as the results state. If you have any
> suggestions or other methods that 
> could be tried it would be really appreciated.
> Thanks in advance,
> Charnell Jones, HTL(ASCP)
> Compunet Clinical Laboratories
> Dayton, OH
> 
>
 Hi Charnell,
 We make up our own Twort for our Lillie-Twort
modified gram stain.
We have increased the strength of the stain from the
original procedure(Pathologists preference) so you may
need to adjust.

Modified Twort stain--Stock                original
 Neutral red (C.I. #50040)   0.6gm         (0.36gm)
 Fast green  (C.I. #42053)   0.2gm         (0.04gm)
    Mix with 200ml 100% ETOH
Procedure says stock solution is good for 1-3 months.
I don't agree, I make up fresh each time I need to
make more working solution (once a month)

Twort working solution
 1 part stock: 2 parts distilled water
Filter AFTER dilution. Ready to use.


=====
Kathy Wortham
USDA,Food Safety Inspection Service
950 College Station Road
Athens, Ga 30605
(706)546-3556
kjwortham@yahoo.com
__________________________________________________
Do You Yahoo!?
Talk to your friends online with Yahoo! Messenger.
http://im.yahoo.com



<< Previous Message | Next Message >>