IMEB's modified Brown & Brenn kit is used to stain gram pos & neg bacillus.
This kit does not use picric acid in the procedure.  the result is an easy
consistent staining of bacillus in paraffin sections & smears.  Background
color is yellow.   Also included in kit is control slides-one stained 3
unstained!  For more info call (708)780-9650.
-----Original Message-----
From: CEJones0476@aol.com <CEJones0476@aol.com>
To: histonet@pathology.swmed.edu <histonet@pathology.swmed.edu>
Date: Wednesday, February 02, 2000 10:20 PM
Subject: Twort Stain


>Does anyone use the Twort stain by Newcomer Supply? If you haven't heard of
>this procedure it is a variation of the Brown and Hopps that eliminates the
>use of picric acid acetone. The lab I work for switched to this procedure
for
>various reasons, but we are having problems with consistency and the
>pathologists are not satisfied. Although the gram negative and gram
positive
>bacteria are staining appropriately, the background has never been light
>green as the results state. If you have any suggestions or other methods
that
>could be tried it would be really appreciated.
>Thanks in advance,
>Charnell Jones, HTL(ASCP)
>Compunet Clinical Laboratories
>Dayton, OH
>