Re: Pollak's Trichrome?
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From: | "J. A. Kiernan" <jkiernan@julian.uwo.ca> |
To: | "Joan C. Yonchek" <yonchjc@ORTHO.UFL.EDU> |
Reply-To: | |
Content-Type: | TEXT/PLAIN; charset=US-ASCII |
On Thu, 3 Feb 2000, Joan C. Yonchek wrote:
> Was asked to find a source and protocol for a cytology stain called
> Pollak's Trichrome. Years ago it was marketed by Paragon in
> New York. If this is no longer available can someone suggest a
> substitute.
It is in Presnell & Schreibman (1997) Humason's Animal Tissue
Techniques, 5th edn, pp 144-145. Baltimore: Johns Hopkins
Univ. Press. It's cited from a 1944 paper. This is a one-step
trichrome with 4 dyes: acid fuchsine, ponceau 2R, light
green SF and orange G, with PTA, PMA and acetic acid, made up
in 50% alcohol. On page 131 of the same book there some useful
practical comments, recommendations and references about
one-step trichromes, and Pollak's isn't recommended.
My favourite one-step trichrome is Gabe's, which uses amaranth,
fast green FCF and martius yellow, with PMA. It doesn't stain
too darkly, and you can use 10-12 um sections, which are better
for many purposes than thinner ones.
John A. Kiernan,
Department of Anatomy & Cell Biology,
The University of Western Ontario,
LONDON, Canada N6A 5C1
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