We have used Novacastra's Myosin Heavy Chain Slow and Fast (NCL-MHCs &
NCL-MHCf) on paraffin sections of muscle with some success. Basically,
antigen retrieve with pH6 citrate buffer for 10 mins and then incubate with
primary ab overnight at r.t., and visualise with Vector Elite ABC kit.
Contact me if you want more info.

Bob Quilty
Dept. of Neuropathology
Frenchay Hospital
Bristol. UK



----- Original Message -----
From: George King <pat055@abdn.ac.uk>
To: Histonet <histonet@Pathology.swmed.edu>
Sent: Monday, February 14, 2000 11:32 AM
Subject: Muscle antibodies


> Hi there
>
> I am currently looking for antibodies against fast and slow myosin which
> will work on formalin fixed paraffin embedded human sections and would
> appreciate any information concerning suppliers, antigen retrieval
> method, etc.
>
> Thank you in advance for all replies.
>
> --
> George King
> University of Aberdeen
> Department of Pathology
> Foresterhill
> Aberdeen, AB25 2ZD
> Scotland
> Tel. (0)1224 681818 x52842
> Fax. (0)1224 663002
>
>
>