Re: ISH of VEGFs and their receptors
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From: | Agripina Suarez <ASuarez@mrl.ubc.ca> |
To: | Tony Henwood <henwood@mail.one.net.au> |
Reply-To: | |
Content-Type: | |
Dear Tony,
Thanks for your suggestions. I will try the different suggestions
from fellow histonetters and hope to succeed one of these days.
Agripina
From: "Tony Henwood" <henwood@mail.one.net.au>
To: Histonet@pathology.swmed.edu,
"Agripina Suarez" <ASuarez@mrl.ubc.ca>
Date sent: Tue, 8 Feb 2000 00:16:59 +0000
Subject: Re: ISH of VEGFs and their receptors
Priority: normal
> Dear Agripina,
> >
> > Histonetters, help!!!
> > I have been trying to do in situ hybridization of VEGFs (A, B, C, D)
> > and their receptors (Flt-1, -4 and Flk-1) in formalin-fixed, paraffin-
> > embedded normal human vascular tissues and transplant heart
> > vessels but still unsuccessful so far.
>
>
> Try:
> Hybridisation at room temp,
> Remove formamide from the probe solution as well as the stringency
> washings
> After hybridisation, wash in 2xSCC for 5 min only (donot try high and
> low stringency washings).
> Once you get some results, look at your technique and add the above
> steps only if your results are not specific.
>
> Hope this helps,
> Tony
>
> Tony Henwood
> Senior Scientist
> Anatomical Pathology
> Royal Prince Alfred Hospital
> Sydney, AUSTRALIA
>
> http://www2.one.net.au/~henwood
> http://www.pathsearch.com/homepages/TonyHenwood/default.html
Agripina C. Suarez
Cardiovascular Research Laboratory
Room 292, McDonald Research Wing, St. Paul's Hospital
1081 Burrard St., Vancouver
B.C., Canada V6Z 1Y6
Phone: (604) 806-8659
Fax: (604) 806-8351
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