Re: ISH of VEGFs and their receptors

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From:Agripina Suarez <ASuarez@mrl.ubc.ca>
To:Tony Henwood <henwood@mail.one.net.au>
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Dear Tony,
Thanks for your suggestions. I will try the different suggestions 
from fellow histonetters and hope to succeed one of these days. 
Agripina

From:           	"Tony Henwood" <henwood@mail.one.net.au>
To:             	Histonet@pathology.swmed.edu,
             	"Agripina Suarez" <ASuarez@mrl.ubc.ca>
Date sent:      	Tue, 8 Feb 2000 00:16:59 +0000
Subject:        	Re: ISH of VEGFs and their receptors
Priority:       	normal

> Dear  Agripina,
> > 
> > Histonetters, help!!!
> > I have been trying to do in situ hybridization of VEGFs  (A, B, C, D) 
> > and their receptors (Flt-1, -4 and Flk-1) in formalin-fixed, paraffin-
> > embedded normal human vascular tissues and transplant heart 
> > vessels but still unsuccessful so far.
> 
> 
> Try:
> Hybridisation at room temp,
> Remove formamide from the probe solution as well as the stringency 
> washings
> After hybridisation, wash in 2xSCC for 5 min only (donot try high and 
> low stringency washings).
> Once you get some results, look at your technique and add the above 
> steps only if your results are not specific.
> 
> Hope this helps,
> Tony
>  
> Tony Henwood
> Senior Scientist
> Anatomical Pathology
> Royal Prince Alfred Hospital
> Sydney, AUSTRALIA
> 
> http://www2.one.net.au/~henwood
> http://www.pathsearch.com/homepages/TonyHenwood/default.html


Agripina C. Suarez
Cardiovascular Research Laboratory
Room 292, McDonald Research Wing, St. Paul's Hospital
1081 Burrard St., Vancouver
B.C., Canada V6Z 1Y6
Phone: (604) 806-8659
Fax:   (604) 806-8351 



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