Re: IHC staining and animal tissue

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From:amos brooks <>, histonet <>
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<!doctype html public "-//w3c//dtd html 4.0 transitional//en"> <html> Hi, <br>        "<i>The warm soapy rinse????????</i>" <br>    A warm soapy water soak (briefly) will make dry tissues less crumbly, as does an ammonia soak. Caution should be used not to oversoak as this will cause swelling of the tissue and may be linked to the pale nuclei artifact. <br>Amos Brooks <p> wrote: <blockquote TYPE=CITE>If the tissues are Zinc Fixed and placed on plus slides they will likely <br>fall of unless sufficiently washed in H20 after fixation, prior to <br>subsequent processing.  The warm soapy rinse????????</blockquote> </html>
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