> Prentice <lprent@itsa.ucsf.edu> 02/03 12:38 PM >>>
> Hi
>
> I have a project using mouse embryos that have been injected with Evan's
> Blue. Can I safely process these in paraffin or would I be safest with
> frozen sectioning?  I'm afraid of loosing some of the dye in the alcohol
> and toluene steps of paraffin processing.
>
> Thanks, in advance
> Linda
>
> Linda Prentice
> Department of Anatomy
> University of California San Francisco
> 513 Parnassus Ave
> Room S-1343
> San Francisco CA 94143-0452
> Phone 415-476-1439
> Fax 415-476-4845

Linda:

     Have a look at J. Neurocytology 10:607-643, 1981. The authors used Evan's blue with LM, EM and LM fluorescence and were able to fix the dye in the tissue so as to avoid its loss.

Geoff
--
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Geoff McAuliffe, Ph.D.
Neuroscience and Cell Biology
Robert Wood Johnson Medical School
675 Hoes Lane, Piscataway, NJ 08854
voice: (732)-235-4583; fax: -4029
mcauliff@umdnj.edu
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