Linda,
            As far as our group knows you can't use Evens blue it won't hold up to processing, we have used India Ink on samples that are processed in the VIP ( for paraffin) and you can see the stain. 

FYI

Jamie


Jamie Erickson
Associate Scientist
Genetics Institute
1 Burtt Rd
Andover, MA 01810
(978) 247-1348
FAX (978) 247-1333

>>> Linda Prentice <lprent@itsa.ucsf.edu> 02/03 12:38 PM >>>
Hi

I have a project using mouse embryos that have been injected with Evan's
Blue. Can I safely process these in paraffin or would I be safest with
frozen sectioning?  I'm afraid of loosing some of the dye in the alcohol
and toluene steps of paraffin processing.

Thanks, in advance
Linda

Linda Prentice
Department of Anatomy
University of California San Francisco
513 Parnassus Ave
Room S-1343
San Francisco CA 94143-0452
Phone 415-476-1439
Fax 415-476-4845