Victoria,

The TUNEL assay is not a hybridization assay, that is it does not identify a 
specific sequence of nulceotides.

It is an in situ procedure in that it is done on a tissue section rather 
than in a test tube.

In the TUNEL assay a pool of labeled nucleotides is reacted by catalysis 
with single stranded DNA "sticky ends" produced by apoptosis enzymes.

A positive result indicates apoptosis enzymes have cleaved the DNA of the 
cell.

We are doing the ISH TUNEL assay using the R&D Systems kit. Works great.

http://www.rndsystems.com

Tim Morken, B.A., EMT(MSA), HTL(ASCP)
Infectious Disease Pathology
Centers for Disease Control
MS-G32
1600 Clifton Rd.
Atlanta, GA 30333
USA

email: tim9@cdc.gov
       timcdc@hotmail.com

Phone: (404) 639-3964
FAX:  (404)639-3043


----Original Message Follows----
From: Victoria Baker <vbaker60@yahoo.com>
To: HistoNet Server <HistoNet@Pathology.swmed.edu>
Subject: Apoptosis/Tunel by In-Situ
Date: Thu, 10 Feb 2000 04:11:55 -0800 (PST)

Hi Everyone!

I received a protocol that does Apoptosis/Tunel by
In-situ methods.  Yesterday I was in a meeting and
brought this up to my director and 2 other PhD's.
They told me that I was wrong.  I have the one
protocol, its from Intergen I think (I'm at home and
the article is at work).  What I was wondering is if
anyone else is doing this, and which protocol they
felt was better.

Any information would be very appreciated.

Vikki Baker, HT (ASCP)
American Health Foundation
Valhalla, New York

Phone:  914-789-7127



__________________________________________________
Do You Yahoo!?
Talk to your friends online with Yahoo! Messenger.
http://im.yahoo.com







______________________________________________________
Get Your Private, Free Email at http://www.hotmail.com