Re: Air Bubbles

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From:"Tony Henwood" <>,
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Dear Jazz,

> I would like to describe a problem that we have been experiencing in our lab. 
>  The problem that we are experiencing is with our Leica CV5000 
> (auto-coverslipper):
> *  No bubbles are seen in slides after coverslip.
> *  After a day or two, a huge air bubble (gap) is formed, surrounding the 
> tissue specimen.
> *  We have increased the volume of the mounting media, but this causes an 
> overflow of media on top of the cover glass.
> Question:  What is your current use of mounting media, and your use of 
> clearing agent for your staining?
Could it be that the mountant media contains too much xylene?

Regards, Tony 
Tony Henwood
Senior Scientist
Anatomical Pathology
Royal Prince Alfred Hospital

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