Re: Air Bubbles
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From: | "Tony Henwood" <henwood@mail.one.net.au> |
To: | histonet@pathology.swmed.edu, Jazz1522@aol.com |
Reply-To: | |
Content-Type: | text/plain; charset=US-ASCII |
Dear Jazz,
> I would like to describe a problem that we have been experiencing in our lab.
> The problem that we are experiencing is with our Leica CV5000
> (auto-coverslipper):
>
> * No bubbles are seen in slides after coverslip.
>
> * After a day or two, a huge air bubble (gap) is formed, surrounding the
> tissue specimen.
>
> * We have increased the volume of the mounting media, but this causes an
> overflow of media on top of the cover glass.
>
> Question: What is your current use of mounting media, and your use of
> clearing agent for your staining?
>
Could it be that the mountant media contains too much xylene?
Regards, Tony
>
Tony Henwood
Senior Scientist
Anatomical Pathology
Royal Prince Alfred Hospital
Sydney, AUSTRALIA
http://www2.one.net.au/~henwood
http://www.pathsearch.com/homepages/TonyHenwood/default.html
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