Re: 2 Waxy questions
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From: | "Tony Henwood" <henwood@mail.one.net.au> |
To: | "'Histonet'" <histonet@pathology.swmed.edu> |
Reply-To: | |
Content-Type: | text/plain; charset=US-ASCII |
Dear Scott,
>Scenario #1 A rep. comes into your lab with 10-20
>lbs of new paraffin and would like for you to try it.
>What would you be thinking?
>Scenario #2 Instead of 10-20 lbs of paraffin, the
>rep. has about 7 blocks already embedded with their
>new paraffin and would like for you to try it.
>What would you be thinking?
Frst step:
Melt some of the wax and make a block out of it. Try to section it.
Caveats: Some waxes seem to need to "mature" (ie heated for some
12-24 hours) before use in embeding.
Second step:
Process paired slices of liver, spleen, uterus, colon, brain and
lymph node to infiltration stage. Infiltrate one of each pair in
trial wax and current preferred wax for 2 hours, changing once.
Embed in relevant wax.
Gauge ease of sectioning especially uterus, 2um sections of lymph
node, 6-7um sections of brain, any folding of the muscle layers of
the colon, any cracking of the spleen sections, continuity of
hepatocytes in liver.
Third Step:
Does wax dissolve easily in the deparafinisation solutions (xylene
or citric-type oils).
Does the wax interfere with any stains (Retic, trichrome, mucin etc)
or a selection of IPX stains (eg Vimentin, Keratin, LCAg).
A good project for junior staff??
Tony Henwood
Senior Scientist
Anatomical Pathology
Royal Prince Alfred Hospital
Sydney, AUSTRALIA
http://www2.one.net.au/~henwood
http://www.pathsearch.com/homepages/TonyHenwood/default.html
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