The secret to getting good infiltration of bone with GMA is to start with
very thin sections and agitate while infiltrating.  I use a diamond wire saw
to cut sections from rabbit knees no more than 4 mm thick, a disc saw would
work also.  I use a platform shaker while infiltrating.
Patsy

-----Original Message-----
From: Harvey, Jennifer [mailto:Jennifer_Harvey@URMC.Rochester.edu]
Sent: Friday, January 28, 2000 8:23 AM
To: 'Histonet'
Subject: undecalcified bone-plastics


Histonet, 

I am looking for someone that has experience with embedding undecalicified
bone. The specimen we have are rabbit knees. 5x4x2 mm sections were taken of
the joint and fixed in RHT fixative. We tried to use GMA (Tectnovit 7100) to
enbed. It seems that the bone did not get infiltrated. Someone suggested
using LR White instead since it infiltrated better. Any help would be
appreciated.


Jennifer Harvey 
University of Rochester
jennifer_harvey@urmc.rochester.edu
716-273-4129