RE: staining fresh frozen brain sections
<< Previous Message | Next Message >>
From: | "Barry, Lilith" <Lilith.Barry@nrc.ca> (by way of histonet) |
To: | histonet@histosearch.com |
Reply-To: | |
Content-Type: | text/plain; charset="us-ascii" |
Barry,
My original response WAS for neuronal tissue, but I am sure that the
histoneters will appreciate information on unfixed free-floating
immunohistochemistry.
Thanks again,
Lilith
-----Original Message-----
From: Barry Rittman [mailto:brittman@mail.db.uth.tmc.edu]
Lilith,
call me Barry please, the PhD stands for
"piled higher and
deeper".
Not sure I have a reference to what you asked but I will
look for one. These are
mostly observations from staining lots of sections for IHC
and histochemistry.
My experience to nervous tissue is limited to Bielschowsky
preparations from
almost 40 years ago so hate to give advice on this tissue.
Barry
"Barry, Lilith" wrote:
> Dear Dr. Rittman,
>
> Thank you for the comment on fresh - floating tissue
processing. Could you
> please give some references on this topic. Is it
applicable for neuronal
> tissue ? What about the morphology? I have mainly worked
with brain
> tissue, labeling neuropeptides for light and electron
microscope and
> haven't come across a paper that deals wit it.
>
> Waiting for your usual informative response,
>
> Lilith
<< Previous Message | Next Message >>