Lynn,
my experience with decals has been that it depends on the decal solution,
the length of time the tissue was in decal and which primary antibodies are
being performed.  We use Dako's Target Retrieval Solution, steam for 20
minutes and allow to cool for 20 minutes and have good success.  We also run
a Vimentin as a control whether the doctor has ordered it or not.  That is
just to check the antigenicity level of the tissue.  Good luck

Joe Nocito, B.S., HT(ASCP)QIHC
Histology Supervisor
Christus Santa Rosa Hospitals
San Antonio, Texas


> -----Original Message-----
> From:	Lynn Gardner [SMTP:lynn-gardner@uiowa.edu]
> Sent:	Monday, January 31, 2000 10:23 PM
> To:	Histonet@pathology.swmed.edu
> Subject:	immuno on decaled tissue
>
> Dear all,
>
>  I would like to know what people are doing to perform immuno on tissues
> that have been decalcified. First of all is it possible and if so do you
> antigen retrieve and perform the same protocol as regular tissue or are
> there special things you should do to the tissue before staining?
>
> Also, if anyone has a tried and true protocol for this I would appreciate
> a
> copy you can fax it to 1-319-335-7770, please make sure to address it to
> me
> as this is a community fax machine.
>
> As always thanks everyone in advance!
>
> Lynn Gardner, HT(ASCP)
>