It depends on what you are going to do after freezing. You don't have to fix
them, if you are going to cryosection them. You might want to freeze them in
tissueteck... Need more details to help you.

Lilith
- - - - - - - - - - - - - - - - - - - -

Lilith Ohannessian-Barry
National Research Council
Institute of Biological Sciences
CANADA
Tel;613-993-6460
Fax;613-941-4475
e-mail; lilith.barry@nrc.ca <mailto:lilith.barry@nrc.ca>


		-----Original Message-----
		From:	Sally Prouty [mailto:ProutyS@physiology.uiowa.edu]
		Sent:	Monday, January 31, 2000 12:11 PM
		To:	Histonet Group (E-mail)
		Subject:	freezing mice embryos

		Hi,
		 I have a few questions about freezing mouse embryos. Do you
have to fix the
		embryo before you freeze it? Or can we just freeze it in
Liquid Nitrogen
		cooled isopentane? If you have to fix the embryo's what kind
of fixative is
		best to use and for how long?


		Thank you,

		Sally Prouty
		HHMI/UIOWA
		sally-prouty@uiowa.edu