I would suggest 10% NBF.  I would have chosen it over Bouins from the start.
Out of curiosity, what were the advantages of Bouins for eye preparation? I
would fix the enucleated eye in 20 to 25 times its volume in formalin.
Years ago when I worked in a veterinary school we would process eyes per the
procedure in the AFIP staining manual (3rd edition).  The reference in the
AFIP manual is to a paper written by Ballou in the American Journal of
Medical Technology, 1966.  That is an excellent paper and worth the read for
anyone doing eye work.

Rob

-----Original Message-----
From: Raverty, Stephen AGF:EX [mailto:Stephen.Raverty@gems3.gov.bc.ca]
Sent: February 13, 2000 2:53 PM
To: 'Histonet@pathology.swmed.edu'
Subject: eye fixation


Due to concerns regarding Bouin's, our lab is currently revising procedures
for fixation of eyes collected from domestic livestock and pet animals.
Would anyone in the list have suggestions for alternative fixatives?
	Thank you,
	S Raverty
	Veterinary Pathologist
	BCMAF, Abbotsford, BC