RE: Processing protocol for breast specimens

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From:"Weems, Joyce" <>
To:"'Scott, Allison D'" <>,
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Some of the pointers (all collected from the net) is to put a xylene between
the last two 100% alcohols on the processor. This helped a lot! Then, if the
tissue is still raw - press it between paper towels to extract as much
moisture as possible and let it set in paraffin for a while to reinfiltrate.
This has saved quite a bit of reprocessing. 
Joyce Weems
Pathology Manager
Saint Joseph's Hospital of Atlanta

	-----Original Message-----
	From:	Scott, Allison D []
	Sent:	Monday, February 07, 2000 4:27 PM
	Subject:	Processing protocol for breast specimens

	Does anyone have a protocol for processing breast specimens.  We get
	cases at least 2 to 3 times a week.  Our pathologist is not very
happy about
	the turnaround time in getting the case out.  Our first problem is
that the
	tissues were cut in to big and thick. our residents donot listen to
	or follow instructions very well.  So therefore we have to work with
	very big fatty pieces of tissue.  We are putting the cassettes into
	Fixx solution(fixative for fatty tisue), for overnight fixation.
The next
	day, they are put into methanol for 2 to 3 hours. After the
methanol, we put
	them into acetone for the remainder of the day.  They are then
loaded on the
	VIP for 2 changes of formalin, 2 changes of 95% alcohol, 4 changes
of 100%
	alcohol, clear rite 3 and then paraffin.  These are for 1 hour each.
	some instances we still have to run some cassettes back because they
	still not  processed well. If someone has a suggestion please

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