I am sooo glad we are not the only ones with a love/hate (mostly hate?)
relationship with our Hacker coverslipper!




At 09:06 AM 2/7/2000 -0800, Carson, Karla wrote:
>Tony,  We are having the same problems (part of the time) with our Hacker
>coverslipprer.  We think we have it fixed and then it pops up its ugly head
>again.  Most of the time I would like to throw it away and go back to hand
>coverslipping.  If you get any good solutions let us know.
>Karla Carson HT/HTL (ASCP)
>Regional Pathology Supervisor
>Mercy Health Care Sacramento
>e-mail kcarson@chw.edu <mailto:kcarson@chw.edu> 
>
>		-----Original Message-----
>		From:	Tony Henwood [mailto:henwood@mail.one.net.au]
>		Sent:	Monday, February 07, 2000 4:17 PM
>		To:	histonet@pathology.swmed.edu; Jazz1522@aol.com
>		Subject:	Re: Air Bubbles
>
>		Dear Jazz,
>
>		> I would like to describe a problem that we have been
>experiencing in our lab. 
>		>  The problem that we are experiencing is with our Leica
>CV5000 
>		> (auto-coverslipper):
>		> 
>		> *  No bubbles are seen in slides after coverslip.
>		> 
>		> *  After a day or two, a huge air bubble (gap) is formed,
>surrounding the 
>		> tissue specimen.
>		> 
>		> *  We have increased the volume of the mounting media, but
>this causes an 
>		> overflow of media on top of the cover glass.
>		> 
>		> Question:  What is your current use of mounting media, and
>your use of 
>		> clearing agent for your staining?
>		> 
>		Could it be that the mountant media contains too much
>xylene?
>
>		Regards, Tony 
>		> 
>		Tony Henwood
>		Senior Scientist
>		Anatomical Pathology
>		Royal Prince Alfred Hospital
>		Sydney, AUSTRALIA
>
>		http://www2.one.net.au/~henwood
>		http://www.pathsearch.com/homepages/TonyHenwood/default.html
>		
>


**************************************************************************
Jill Songer HT (ASCP)
Anatomic Pathology Supervisor
Virginia Tech 
Veterinary Medical Teaching Hospital