To make 0.2N Hydrochloric Acid
Use ACS grade Hydrochloric acid ( ie:  EM Science part # HX0603-3 ) dilute
16.1ml per liter in a class "A" volumetric flask

To Standardize:  20 ml sample is titrated with freshly factorized 0.1N Sodium
Hydroxide by using a few drops of Phenolphthalein indicator as per : 1ml 0.2N
Hydrochloric Acid = 2 ml 0.1N Sodium Hydroxide

Final Test : Using NIST SRM 723, TRIS >> TRIS is titrate potentiomentrically
with HCL 0.2N sample using Methyl Red as indicator as per: Normality HCL = (wt
in grams of TRIS )  * (%of SRM purity) / (vol in ml of sample used )
* (12.114 )
The reference for standardization is ASTM E 200-91
Colour testing: APHA 2120B  -visual comparison
or
You can buy ready made Hydrochloric Acid ready made from VWR  1-800-932-5000
Hydrochloric Acid solution 0.2000 N      + 0.0004N
CAT #
VW3593-1 =  1L
VW3593-4  = 4L
VW3593-7 = 20L
APHA cover page, NIST traceable, colour limit 10 =max

Regards
Gordon Fenn
BDH/ EM Science Toronto






"Smith, Allen" <asmith@mail.barry.edu> on 02/13/2000 03:01:15 PM

To:   "'histonet@pathology.swmed.edu'" <histonet@pathology.swmed.edu>
cc:    (bcc: Gordon Fenn/BDH/EMI/Merck)
Subject:  FW: My brain is fried and I need help





-----Original Message-----
From:     Jennings-Siena, Debbie [mailto:ds.jennings-siena@baylordallas.edu]
<mailto:[mailto:ds.jennings-siena@baylordallas.edu]>
Sent:     Friday, February 11, 2000 6:16 PM
To:  'histonet@pathology.swmed.edu'
Subject:  My brain is fried and I need help

Ok everyone,
You can laugh at me later but I really need your help with this math
question.   I need to make up a 0.2N solution of HCL.  Our HCL is 36.6 to
38% and the FW is 36.46.  Help please.
Debbie J. Siena
The "concentrated hydrochloric acid" sold by chemical companies is 12 N
(really somewhere between 11.8 N and 12.2 N).
0.2 is one sixtieth of 12.  Thus 1 ml concentrated hydrochloric acid plus 59
ml water is a 0.2 N.  If you need a lot of 0.2 N hydrochloric acid, you can
add 16.5 ml of concentrated hydrochloric acid to 983.5 ml water (or add
enough water to 16.5 ml concentrated hydrochloric acid to make 1 liter of
total solution).
Shugar & Ballinger [Chemical Technician's Ready Reference Handbook,
McGraw-Hill, 1990] say to use 16.4 ml concentrated hydrochloric acid for 1
liter of 0.2 N.  This is true only if you have a new bottle of the stuff.
               Allen A. Smith
               School of Graduate Medical Sciences
               Barry University
               Miami Shores, FL  33161