Human CD3 cells!

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     Hi to All!  I have a question concerning staining of human CD3 cells.
Someone in our department is interested in staining "living cells" in a gel. In
summary, the antibody would need to be directly conjugated (preferably with an
enzyme) and able to diffuse through the gel to stain the cells.  Is anyone
of a conjugated antibody that could accomplish this??  We found a rabbit anti
human CD3 (Dako) but it reacts with the intracytoplasmic portion of the CD3
antigen; thus  this particular antibody is not appropriate.  Please respond
privately if you prefer. Thank you so much as always for sharing your

Jennifer Hoover
Novartis Pharmaceuticals

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