Cyclin D1 -Reply
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From: | Richard Cartun <Rcartun@harthosp.org> |
To: | JKoutny@cytometry.com, Histonet@pathology.swmed.edu |
Reply-To: | |
Content-Type: | text/plain; charset=US-ASCII |
We, too, use the DAKO mAb (M7155) for cyclin D1 detection. Unlike most of our IP stains that are now performed with EnVision+, we continue to use DAKO's LSAB+ for cyclin D1 (one of my hematopathology colleagues likes the immunoreactivity with LSAB+ better than that with EnVision+). Personally, I think the secret (as usually) is having well fixed and processed tissue, as well as prolonged heat treatment. We pretreat our tissue sections with DAKO's "High pH" retrieval solution in a 98 degree waterbath for forty (40) minutes. In addition, we incubate with the mAb diluted 1:1,000 overnight at RT. You can also incubate 30 minutes but you will have to decrease the dilution to approximately 1:200. If you continue to have problems please contact me and I can send you some of our positive control sections.
R. Cartun
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