CD23 -Reply

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From:"Nader, Alexander" <>
To:histonet <>
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We use a CD23-antibody from Novocastra, with consistent and good results. 10
min epitope retrieval in a household-steamer, citrate buffer pH 6, 1:40,
followed by DAKO's dedection kit.

Dr. Alexander Nader
Path. Institut Hanuschkrankenhaus
Vienna, Austria (No, I didn't vote for HIM!) <>

> We are currently looking at two anti-CD23 mAbs; one from DAKO 
> (M0763) and one from the Binding Site (MH112).  The one from 
> DAKO is not listed as "Applicable to formalin-fixed tissue" 
> unless their CSA detection is used; however, we have been 
> able to obtain good immunoreactivity following HIER in a 98 
> degree waterbath and then detection with DAKO's EnVision+.

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