We use a CD23-antibody from Novocastra, with consistent and good results. 10
min epitope retrieval in a household-steamer, citrate buffer pH 6, 1:40,
followed by DAKO's dedection kit.

Dr. Alexander Nader
Path. Institut Hanuschkrankenhaus
Vienna, Austria (No, I didn't vote for HIM!)
 
Alexander.Nader@wgkk.sozvers.at <mailto:Alexander.Nader@wgkk.sozvers.at>
privat: Alexander.Nader@univie.ac.at
<mailto:Alexander.Nader@univie.ac.at> 

> We are currently looking at two anti-CD23 mAbs; one from DAKO 
> (M0763) and one from the Binding Site (MH112).  The one from 
> DAKO is not listed as "Applicable to formalin-fixed tissue" 
> unless their CSA detection is used; however, we have been 
> able to obtain good immunoreactivity following HIER in a 98 
> degree waterbath and then detection with DAKO's EnVision+.