Hi to All!  I just have a question concerning Acetone fixation of frozen
tissue, especially concerning spleen.  Could you please share your protocols and
other special tricks of the trade?  Currently whether I fix at 4C or -20C for 3
or 5 minutes the Acetone seems to almost chew up the spleen on the slide!  I
have not encountered this problem in the past with other tissue types.  Any
suggestions would be very much appreciated.  Thank you for sharing the
knowledge!


Jennifer Hoover
Novartis Pharmaceuticals