gallyas stain

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From:Shelley Sheridan <> (by way of histonet)
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Hello all.
I am working to develop the Gallyas stain for
neuropathology.  We normally use immunohistochemistry
for our diagnostics, so we have very little experience
with special stains.  We are having problems with the
developer reaction.  We know that when you add
together the two developer solutions the mixture will
turn cloudy and then go clear.  Our solution is
staying cloudy, even though we use acid washed
glassware and very clean water.  The cloudy mixture is
developing the sections, but the staining is not
ideal.  We would like to get the developer reacting
correctly before we start using the stain on more
important tissue.  Does anyone have any ideas?  How
slowly do you add the solutions together?  Do you use
a separation funnel?  Is there some special technique
to making the developer that we don't know about?
Thank you ahead of time for your help.

Shelley K. Sheridan
Research Specialist
Center for Neurodegenerative Disease Research
University of Pennsylvania Medical School
Philadelphia, PA 19104
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