fixation of cells for immunostaining
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From: | David Grehan <david.grehan@olhsc.ie> (by way of histonet) |
To: | histonet@histosearch.com |
Reply-To: | |
Content-Type: | text/plain; charset="us-ascii" |
Hi,
Does anyone have a good protocol for fixing and storing cell
preparation following cytospin or touch prep which will be subsequently
immunostained. I have tried a number of methods but with indifferent
results. I work in a paediatric hospital so we do not get many cytology
specimens to do immunocytochemistry, but occasionally it would be useful
to have a good reliable protocol. Is 50:50 methanol acetone a good
fixative and should I air dry before fixation or not.
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