Re: gallyas stain
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From: | Mary Latimer <ml4@st-andrews.ac.uk> (by way of histonet) |
To: | histonet@histosearch.com |
Reply-To: | |
Content-Type: | text/plain; charset="us-ascii" |
Dear Shelley I have done the Gallyas Method for Myelin for a number of
years the solution should be clear I suggest that you make the solutions
from analytical grade salts ...there are also 3 solutions to add together
sodium carbonate, silver with and without formaldyhyde.The most likely
problem is the water I use fresh distilled this stain can be quite
difficult but very rewarding good luck
On Thu, 16 Dec 1999, Shelley Sheridan wrote:
>
> Hello all.
> I am working to develop the Gallyas stain for
> neuropathology. We normally use immunohistochemistry
> for our diagnostics, so we have very little experience
> with special stains. We are having problems with the
> developer reaction. We know that when you add
> together the two developer solutions the mixture will
> turn cloudy and then go clear. Our solution is
> staying cloudy, even though we use acid washed
> glassware and very clean water. The cloudy mixture is
> developing the sections, but the staining is not
> ideal. We would like to get the developer reacting
> correctly before we start using the stain on more
> important tissue. Does anyone have any ideas? How
> slowly do you add the solutions together? Do you use
> a separation funnel? Is there some special technique
> to making the developer that we don't know about?
> Thank you ahead of time for your help.
>
>
>
>
>
>
> =====
> Shelley K. Sheridan
> Research Specialist
> Center for Neurodegenerative Disease Research
> University of Pennsylvania Medical School
> Philadelphia, PA 19104
> Phone:(215)614-0051
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