Re: amplification kit

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From:Katri Tuomala <> (by way of histonet)
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Hi Klazien,
You may have received the answer to your inquiry already, but I have not
seen it. I have used this kit on paraffin sections with antibodies that
are normally not demonstrable in paraffins. I had to do a lot of
tweaking with the antigen retrieval and antibody dilutions, because of
the background staining. I have a feeling you may be successful with a
much higher primary antibody dilution. Do a wide titration first to get
closer to real titre and then narrow it down. Good luck!


Katri Tuomala
Anatomic Pathology
St.Joseph's Hospital
Hamilton, Ontario, Canada

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