Re: amplification kit
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From: | Katri Tuomala <katri@istar.ca> (by way of histonet) |
To: | histonet@histosearch.com |
Reply-To: | |
Content-Type: | text/plain; charset="us-ascii" |
Hi Klazien,
You may have received the answer to your inquiry already, but I have not
seen it. I have used this kit on paraffin sections with antibodies that
are normally not demonstrable in paraffins. I had to do a lot of
tweaking with the antigen retrieval and antibody dilutions, because of
the background staining. I have a feeling you may be successful with a
much higher primary antibody dilution. Do a wide titration first to get
closer to real titre and then narrow it down. Good luck!
Katri
Katri Tuomala
Anatomic Pathology
St.Joseph's Hospital
Hamilton, Ontario, Canada
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