Re: Un-coverslip

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From:Jeffrey S Crews <> (by way of histonet)
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I like to put the slide into xylene for a few hours, then slide the edge
of a razor blade under the edge of the coverslip where the mountant has
become soft. Stop pushing in the blade when it becomes difficult or you
get near the tissue. You can use multiple blades on all edges if you're
in a real hurry. Then put the whole thing back into xylene. The gap made
by the blade edge helps the xylene penetrate under the glass faster. You
can check periodically to see if you can insert the blades further. After
another couple hours the coverglass should come off. The whole process
shouldn't take more than a work day at most.

On Mon, 20 Dec 1999 16:11:38 EST "kunihiro uryu" <>
>Does any one know how to uncoverslip in a relatively quick way from
>mountained by permanent mounting medium?  We use Cytoseal 60 from
>Scientific as mounting medium.  Normally when I need I keep the slide
>to be un-coverslipped.  It could take a few days to a week or more
>how hard it is.  If anyone knows a quick method for uncoverslipping
>share with me, I would greatly appreciate it.
>U of Penn
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