Re: Un-coverslip

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From:"Bro. Lauren Ball" <> (by way of histonet)
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I've had good luck with a hot plate.  Have also used a Bunsen burner in the
past when such things were still allowed.  This also took some practice to
control the heat.  Place slide on a hot plate the labeled end hanging off
the edge to be used  as a handle. Wear glove the slide still gets hot. The
temperature should be about 250 to 280 F.  The heat will soften the mounting
medium, With a dissecting needle on any edge of the coverslip, gently push,
the cover slip will move slowly, when it is off the edge of the slide gently
lift it and it will "Ping" itself off of the slide.  Do not heat to hot or
you will cook the section.  Do not push to hard or you will break the
coverslip.  This is much faster than soaking in xylene.  For "Tape
coverslips" 10 minutes in acetone followed by fresh change of Acetone.
----- Original Message -----
From: Jeffrey S Crews <>
To: <>
Cc: <>
Sent: Monday, December 20, 1999 2:56 PM
Subject: Re: Un-coverslip

> I like to put the slide into xylene for a few hours, then slide the edge
> of a razor blade under the edge of the coverslip where the mountant has
> become soft. Stop pushing in the blade when it becomes difficult or you
> get near the tissue. You can use multiple blades on all edges if you're
> in a real hurry. Then put the whole thing back into xylene. The gap made
> by the blade edge helps the xylene penetrate under the glass faster. You
> can check periodically to see if you can insert the blades further. After
> another couple hours the coverglass should come off. The whole process
> shouldn't take more than a work day at most.
> jc
> On Mon, 20 Dec 1999 16:11:38 EST "kunihiro uryu" <>
> writes:
> >Does any one know how to uncoverslip in a relatively quick way from
> >slide
> >mountained by permanent mounting medium?  We use Cytoseal 60 from
> >Stephens
> >Scientific as mounting medium.  Normally when I need I keep the slide
> >glass
> >to be un-coverslipped.  It could take a few days to a week or more
> >depending
> >how hard it is.  If anyone knows a quick method for uncoverslipping
> >and
> >share with me, I would greatly appreciate it.
> >
> >Hiro
> >U of Penn
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