Re: Cells

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From:Amos Brooks <> (by way of histonet)
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    Next time you do this, instead of  using an agar to culture the cells you
might try an agar broth. Then you could spin the growing cells down and make
them into a cell block, using the button of cells at the bottom of the tube.
Then process the button as you would the tissue you are comparing it to. You
could culture the cells you currently have on agar into a broth, if all else
    Another plan would be to cut out the colonies from the solid agar you have
and place the colony between fine filter papers and process this routinely.
good luck,
Amos Brooks

Lynn Gardner wrote:

> Dear Histonetters,
> First of all Merry Christmas and Happy New Year to all!! I have a question
> that hopefully someone will have an answer.
> We have grown up some cells in a petri dish. We are now wanting to try and
> process these cells, however, we need to keep them in their shape and not
> disrupt them. Does anyone have an idea of how to do this or reference to
> journal articles that would contain this information?
> I thought we could place the cells in agar and then process them but don't
> know what type of agar would process and cut well. Would like any imput
> possible.
> Also, is there anyone out there that knows of a cytology network like our
> histonet?
> Thanks for your help everyone! Happy Holidays!
> Sincerely,
> Lynn Gardner

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