Re: Antigen Retrieval, Vimentin & BrdU

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From:Tom Kuwahara <> (by way of histonet)
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Hi Katie:  I would think the trick is in which one of the substrates
will survive the MW/AR step.  DAB  would be more likely to survive than
the vector red altho you can try it and see.  Since BRDU is in the
nuclei and the vimentin out in the cytoplasm, you could try two colors
of DAB also.  Tom

"Bennett, Catherine (Katie)" wrote:
> One idea that has been posed to me is to do the Vimentin stain first.  Then
> to do the microwave AR, protease, and HCl pre-treatments then the BrdU
> staining.  Might I loose the Vimentin stain if I go in this order?  (I'm
> using a Vector ABC-AP kit and Vector Red as the chromagin for Vimentin and a
> Vector ABC kit with DAB for the BrdU.)
> ****************************
> Catherine "Katie" Bresee Bennett
> Sr. Technical Associate
> Lovelace Respiratory Research Institute
> Albuquerque, New Mexico

Thomas J. Kuwahara
Senior Immunohistochemist
Advanced Pathology Systems
3801 Sacramento St. suite 621
San Francisco, CA 94118
415 750 6800 x23067 tel
415 750 2332 fax

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