RE: fixation of cells for immunostaining
<< Previous Message | Next Message >>
From: | Emma Carter <E.Carter@OxfordBioMedica.co.uk> (by way of histonet) |
To: | histonet@histosearch.com |
Reply-To: | |
Content-Type: | text/plain; charset="us-ascii" |
My colleague calls the methanol acetone mix 'Stinky Fix', and she is
right!!! We use that for in situ on cultured cells.
For immuno on cultured cells i usually fix in acetone, at -20 Celsius,
but i think that is probably from naivety, rather than precision. (self
taught histologist!)
Probably not much help, but my 2 cents worth!
emma carter
Oxford BioMedica (UK) Ltd
> -----Original Message-----
> From: David Grehan
> Sent: Friday, December 17, 1999 1:56 PM
> To: 'histonet'
> Subject: fixation of cells for immunostaining
>
> Hi,
> Does anyone have a good protocol for fixing and storing cell
> preparation following cytospin or touch prep which will be
> subsequently
> immunostained. I have tried a number of methods but with indifferent
> results. I work in a paediatric hospital so we do not get many
> cytology
> specimens to do immunocytochemistry, but occasionally it would be
> useful
> to have a good reliable protocol. Is 50:50 methanol acetone a good
> fixative and should I air dry before fixation or not.
>
<< Previous Message | Next Message >>