RE: VIP 3000 Biopsy Programs

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From:"Weems, Joyce" <> (by way of histonet)
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We have the VIP 1500 dedicated to biopsies - GI, heart, lung, etc. We have:
10% formalin x 2
60% Alcohol (Stephens Blends)
70% Alcohol
80% Alcohol
95% Alcohol
100% Alcohol x 2
Xylene X 2
Paraffin X 4 (58 degrees)

Vacuum on all, 37 degrees C, 10 minutes each. I'm not aware of any problems!

Joyce Weems
Pathology Manager
Saint Joseph's Hospital of Atlanta

	-----Original Message-----
	From: []
	Sent:	Friday, December 17, 1999 4:27 PM
	Subject:	VIP 3000 Biopsy Programs

	Hi Histonetters,
	Soon, we will be bringing on-line a new processor (VIP 3000) in
order to
	begin running over night biopsy programs. So I'm in need of some
	First, does anyone have a good preferably Great biopsy program that
	would like to share? We're still using Good OLE 10% formalin,
ethanol, and
	xylene. Please vendors I don't want to try substitutes or other
	Also, any recommendations regarding the VIP 3000 would be
appreciated. Second
	question, Is everyone using heat, vacuum, pressure on their biopsy
	Third?, Are there any biopsy tissue types (i.e., skins, cervical
leeps or
	cone bxs, etc.) that do not process well on shortened biopsy
programs? 4th ?,
	Is anyone using eosin in their last processing absolute ethanol and
are you
	seeing any staining problems on your immuno stained sections?

	Well, that's about it for now.
	Please feel free to fax, if the info is lengthy.
	Thanks for all the help in advance.

	Joel Benoit
	Cooley Dickinson Hospital
	FAX (413) 582-2946

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