RE: Removal of DAB Chromogen

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From:Cynthia Favara <cfavara@niaid.nih.gov> (by way of histonet)
To:histonet@histosearch.com
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I am not familiar with these antibodies so have a few questions. Is the
staining seen in the same cells? If not I would just do a double stain with
a different substrate say VIP or AEC. I have done double staining using 2
HRP reactions and any residual HRP is removed by a second peroxidase block
and or a second HIER. I suggest this as I know it works in my lab. Other
suggestion would be to use a mild bleach solution which should also remove
the DAB, but I don't know what other efects it may have. Good luck!! and
Happy Holidays!!!
Cynthia Favara
NIAID/RML/LPVD
903 South 4th Street
Hamilton, MT 59840
PH: 406-363-9317
FAX: 406-363-9286




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