RE: Antigen Retrieval, Vimentin & BrdU
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From: | "Bennett, Catherine (Katie)" <cbennett@lrri.org> (by way of histonet) |
To: | histonet@histosearch.com |
Reply-To: | |
Content-Type: | text/plain; charset="us-ascii" |
One idea that has been posed to me is to do the Vimentin stain first. Then
to do the microwave AR, protease, and HCl pre-treatments then the BrdU
staining. Might I loose the Vimentin stain if I go in this order? (I'm
using a Vector ABC-AP kit and Vector Red as the chromagin for Vimentin and a
Vector ABC kit with DAB for the BrdU.)
****************************
Catherine "Katie" Bresee Bennett
Sr. Technical Associate
Lovelace Respiratory Research Institute
Albuquerque, New Mexico
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