RE: Antigen Retrieval, Vimentin & BrdU

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From:"Bennett, Catherine (Katie)" <> (by way of histonet)
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One idea that has been posed to me is to do the Vimentin stain first.  Then
to do the microwave AR, protease, and HCl pre-treatments then the BrdU
staining.  Might I loose the Vimentin stain if I go in this order?  (I'm
using a Vector ABC-AP kit and Vector Red as the chromagin for Vimentin and a
Vector ABC kit with DAB for the BrdU.)

Catherine "Katie" Bresee Bennett
Sr. Technical Associate
Lovelace Respiratory Research Institute
Albuquerque, New Mexico

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