Hif staining

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From:Emma Carter <E.Carter@OxfordBioMedica.co.uk> (by way of histonet)
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Does anyone have any protocols or suggestions for Hif staining?

I want to detect hypoxia in mouse xenograft tumours, and although they
are supposed to have had NITP in them, i cannot find any, and i _know_
they _have_ to be hypoxic!!

Hif is a long shot, but i reckon it is worth a try. Tumours are
currently frozen and sectioned.....

any help would be appreciated!

emma carter
Oxford BioMedica

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