EDTA-NaOH, pH 8.0 retrieval fluid

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From:"Lott, Robert" <Robert.Lott@bhsala.com> (by way of histonet)
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Stock Solution:
	1.86 gm. EDTA (same as used for decalcification)
	5.0   liters DI water

To the above solution, add 2 to 2.5 ml. 2N NaOH.
Adjust solution to pH 8.0 with 1N NaOH or 1N HCl solution.

Reference:   Chan, John K.C.,  Advances in Immunohistochemical Techniques:
Toward Making Things Simpler, Cheaper, More Sensitive, and More
Reproducible.  This author is issuing commentary on: Antigen retrieval
techniques in immunohistochemistry: Comparison of different methods, by
Pileri, SA, et.al.; J Pathol   1997; 183; 116-123.  This article appeared in
Advances in Anatomic Pathology, 1998; Vol. 5, No. 5, pp.314-325.

The authors state, and the commentator agrees, that the above retrieval
solution "produces superior results, especially in comparison with the most
widely used citrate buffer (pH, 6.0)."  They compared 61 specific antibodies
on serial sections which were both untreated and treated with 0.05% protease
XIV at 37 degrees for 5 min., 0.01 M citrate (pH, 6.0), 0.1 M Tris-HCL (pH,
8.0), and 1mm EDTA-NaOH (pH 8.0)

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