paraformaldehyde

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From:Gayle Callis <uvsgc@msu.oscs.montana.edu> (by way of histonet)
To:histonet@histosearch.com
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We store 4% paraformaldehyde, made up in PBS at 4C for long periods
of time, two months or so, without problems.  If any precipitate appears
it is tossed, and fresh is made up, carefully, using heat and stirring.
We never heat over 60C, and it usually goes into solution at 56C within
30 min.  Our EM facitlity warned that overheating PFA when making it up,
can ruin the molecule, so care is taken to control that temp.

For 2% we just dilute it with PBS.

Gayle Callis




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