Removal of DAB Chromogen
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| From: | "Richard Cartun" <Rcartun@harthosp.org> (by way of histonet) |
| To: | histonet@histosearch.com |
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| Content-Type: | text/plain; charset="us-ascii" |
Help!
We had a problem in the lab today where our cytokeratin cocktail
(AE1&AE3/CAM5.2) was put on sections of prostate instead of the requested
cytokeratin 34BetaE12 mAb. Is there any way to remove the DAB reaction
product and re-stain the slides using the correct mAb? If we re-stain the
slides do I have to be concerned with binding of the detection reagents to
the first primary mAb?
R. Cartun
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