I too have had extensive experience with oxidation problems, and have tested
out multiple antibodies (clones- specific ones may have problems where others
do not)
but have seen this in tissue stored in a variety of cicumstances(refrig not
baked, refrig -baked, -20 degrees stored ,4 degrees stored, room temp stored,
I tested antibodies at fresh cut, one week, one month, three months, 6 mos,
12 mos and extraneous slides hanging out in drawers and boxes for years.The
results diminshedover time , to no staining at all. I believe this to be a
clone, and method specific problem.                   dana Dittus
                                       histology supervisor
                                      Abington Memorial Hospital
                                     1-215-281-2609