Re: cooked tissue

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From:Marcia Bentz <mb7x@virginia.edu> (by way of histonet)
To:histonet@histosearch.com
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Hi Angeline,
I've never worked with brain tissue before, and it may be more sensitive,
but since I process all my mouse tissue by hand and without vacuum, I
always leave tissues in paraffin bath overnight to help insure complete
infiltration. Don't know if this helps, but it's my 2 cents.

Marcia

At 10:11 AM 12/10/1999 -0500, Angeline Meadows wrote:
>Hello histo-experts!
>
>I was hoping for any insight you might have in regards to a recent
"screw-up"
>here in the lab.  My boss accidentally left our rat brain tissue sections in
>the warm paraffin overnight.  I am getting some really weird labeling and
want
>to know if this overheating could be the problem.  Has this ever happened to
>any of you or are you aware of problems that could arise?  I'm assuming we
>should just consider them toast - but I wanted check 'the' source before I
>tossed them.
>
>Thanks in advance for all your help!
>
>angeline meadows
>cell biology and anatomy
>medical college of georgia
>meadows@neuro.mcg.edu
>
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