Re: artery section with stainless stent
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From: | "R.Wadley" <s9803537@pop3.unsw.edu.au> (by way of histonet) |
To: | histonet@histosearch.com |
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Content-Type: | text/plain; charset="us-ascii" |
Hi,
Your best, & probably only bet is to embed in resin (araldite) &
take it
off to you local geology lab. There a section can be cut, mounted on a
slide & ground down to a suitable thickness. Then you can stain the
section. This process wastes a lot of tissue, but you should be able to
get 2 - 5 sections per block.
If you are lucky & the stent is extremely fine you may be able (after
embedding in resin) to section with a hardened steel blade. The results
will not be as good as using the grinding & polishing technique, & may
distort the position of the stent, but you can with care get more sections
to look at.
I seem to remember a thread on this sometime ago.
Regards
Rob W.
At 07:53 12/02/1999 +0100, you wrote:
>A friend asked if anyone had suggestions on how to section an artery
>with a stainless steel stent in place. I had not idea, as we had
>tried sectioning dacron blood vessels.
>Would anyone on this list have suggestions on how to approach this?
>Taking the stent out is not an option.
>THANKS!
R. Wadley, B.App.Sc. M.L.S, Grad.Dip.Sc.MM
Laboratory Manager
Cellular Analysis Facility
School of Microbiology & Immunology
UNSW, New South Wales, Australia, 2052
Ph (BH) +61 (2) 9385 3517
Ph (AH) +61 (2) 9555 1239
Fax +61 (2) 9385 1591
E-mail r.wadley@unsw.edu.au
www http://www.micro.unsw.edu.au/caf.html
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