Re: Tyrosine hydroxylase and dopamine Beta hydroxylase

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From:Geoff McAuliffe <mcauliff@UMDNJ.EDU> (by way of histonet)
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"Michael J. Lyon, Ph.D." wrote:

> I have been searching Linscott's for sources of Tyrosine hydroxylase and
> dopamine Beta hydroxylase antibodies and there are just to many sources and
> variations so I thought I would post this inquiry to you more experienced
> netters.  I would like to get some of your experiences with either Tyrosine
> hydroxylase and dopamine Beta hydroxylase antibodies: sources etc.  It would
> be good if these cross react in humans and rats but human reactivity is the
> most important.  Also, the tissues will most likely be formalin fixed some
> whole mount vascular elements as well as frozen sections.
> Thanks
> Mike
> Michael J. Lyon, Ph.D.
> Associate Professor Otolaryngology
> 315-464-7253 Voice
> 315-464-5572  FAX

    I have had great success with antibodies to TH and DBH from Eugene Tech. on
formalin-fixed frozen or cryostat sections of rat or mouse tissues. I use the
Vector or Vector Elite kits for detection.
    My impression is that there are several (or more) companies supplying good
antibodies to these enzymes.

Geoff McAuliffe, Ph.D.
Neuroscience and Cell Biology
Robert Wood Johnson Medical School
675 Hoes Lane, Piscataway, NJ 08854
voice: (732)-235-4583; fax: -4029

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