Re: Tyrosine hydroxylase and dopamine Beta hydroxylase
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From: | Geoff McAuliffe <mcauliff@UMDNJ.EDU> (by way of histonet) |
To: | histonet@histosearch.com |
Reply-To: | |
Content-Type: | text/plain; charset="us-ascii" |
"Michael J. Lyon, Ph.D." wrote:
> I have been searching Linscott's for sources of Tyrosine hydroxylase and
> dopamine Beta hydroxylase antibodies and there are just to many sources and
> variations so I thought I would post this inquiry to you more experienced
> netters. I would like to get some of your experiences with either Tyrosine
> hydroxylase and dopamine Beta hydroxylase antibodies: sources etc. It would
> be good if these cross react in humans and rats but human reactivity is the
> most important. Also, the tissues will most likely be formalin fixed some
> whole mount vascular elements as well as frozen sections.
>
> Thanks
>
> Mike
>
> Michael J. Lyon, Ph.D.
> Associate Professor Otolaryngology
> 315-464-7253 Voice
> 315-464-5572 FAX
I have had great success with antibodies to TH and DBH from Eugene Tech. on
formalin-fixed frozen or cryostat sections of rat or mouse tissues. I use the
Vector or Vector Elite kits for detection.
My impression is that there are several (or more) companies supplying good
antibodies to these enzymes.
Geoff
--
**********************************************
Geoff McAuliffe, Ph.D.
Neuroscience and Cell Biology
Robert Wood Johnson Medical School
675 Hoes Lane, Piscataway, NJ 08854
voice: (732)-235-4583; fax: -4029
mcauliff@umdnj.edu
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