Re: Tissue Adhesion for Immunos

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I have a question.  We use sialanized slides that we dip ourselves for our
immunos,  BUT since we are cutting slides for H&E's also, we use the same
waterbath and it has gelatin in it.  Someone said to never use any other
adhesive in the  waterbath.  My question is "why not"?  We don't have any
trouble with any thing coming off the slides, and don't have any unspecific
staining due to gelatin.
Karen Dulany  HTL(ASCP)
Eppley Institute for Cancer Research
Omaha, NE

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